Fig. 3
(A) Expression of TF on MVs was determined using an APC-conjugated anti-human TF antibody by flow cytometry, with gating based on an unstained sample (data not shown). The horizontal axis represents TF-positive cells, and the vertical axis represents cell count. (B) Western blot analysis of TF expression in AsPC-1 and MIA PaCa-2 cells and MVs (20 µg/well). AsPC-1 TFhigh cell lysate (PC) was used as positive control of TF expression and β-actin was used as internal control. (C) TF activity was measured using a 1-stage clotting assay